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Antibodies bound to the blocking peptide not bind for the epitope about the target protein. By evaluating the staining from your blocked antibody vs . the antibody alone, you can see which staining is specific.
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This tutorial outlines additional doable optimization of cellular and immunoassay parameters to guarantee the best possible benefits are...
This guide outlines more probable optimization of cellular and immunoassay parameters to make certain the very best results are...
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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by the two antibodies...
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Blocking peptides are peptides that bind especially for the goal antibody and block antibody binding. These peptide typically consists of the epitope recognized by the antibody.
Target Information and facts Cyclin E belongs towards the highly conserved cyclin family, whose users are characterised by a spectacular periodicity in protein abundance throughout the cell cycle. Cyclins functionality as regulators of CDK kinases. Different cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E types a posh with and features as being a regulatory subunit of CDK2, whose exercise is needed get more info for cell cycle G1/S changeover. This protein accumulates at the G1-S period boundary and it is degraded as cells development by way of S stage.
Overexpression of the gene has become observed in several tumors, which results in chromosome instability, and thus may possibly contribute to tumorigenesis. This protein was identified to affiliate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped on the ATM locus), which participates in mobile-cycle regulated histone gene expression and performs a crucial job in marketing mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode unique isoforms, are actually explained. Two further splice variants were being noted but specific nucleotide sequence information is just not nonetheless obtainable.
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